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ASCP ASCP-MLT : MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
Exam Code: ASCP-MLT
Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
Updated: Sep 27, 2026
Q & A: 562 Questions and Answers
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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Immunology and Serology | 5-10% | - Infectious disease serology - Immune system principles and components - Autoimmune and immunodeficiency disorders - Immunologic testing methodologies |
| Clinical Chemistry | 20-25% | - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring - Toxicology and liver/kidney function tests - Electrolytes, acid-base balance, and blood gases |
| Laboratory Operations | 5-10% | - Pre-analytical, analytical, and post-analytical processes - Laboratory mathematics and instrumentation - Quality control and quality assurance - Laboratory safety and regulatory compliance |
| Blood Banking (Immunohematology) | 15-20% | - Blood group systems and antigens - Antibody detection and identification - Transfusion reactions and adverse events - Blood components and storage - Compatibility testing |
| Hematology | 20-25% | - Red blood cell physiology and disorders - White blood cell morphology and diseases - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis - Urine formation and composition - Cerebrospinal, serous, and synovial fluids - Semen and amniotic fluid testing |
| Microbiology | 15-20% | - Mycology, parasitology, and virology - Identification of pathogenic microorganisms - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question #1
The FTA-ABS is used to confirm that a positive non-treponemal test like RPR is not the result of a biological false positive, which occur in about 1 to 10 percent of the population.
A positive RPR test and a negative FTA-ABS test is most likely the result of:
- A. Secondary syphilis
- B. False positive reaction
- C. Primary syphilis
- D. Latent syphilis
Correct Answer: B 🗳️
Question #2
G6PD deficiency causes an increased suseptibility to the oxidation of hemoglobin, which in turn, causes the precipitation of hemoglobin inside of the red blood cell.
Which one of the following conditions is associated with glucose-6-phosphate dehydrogenase (G6PD) deficiency?
- A. Precipitation of hemoglobin
- B. Microcytic red cells
- C. Hemoglobins with low oxygen affinities
- D. Faulty heme synthesis
Correct Answer: A 🗳️
Question #3
Confirmed cases of H1N1 virus have been reported more often in children and younger adults than in any other age group.
Micro
The age groups that have been most affected by the H1N1 virus are children and younger adults.
- A. false
- B. true
Correct Answer: B 🗳️
Question #4
The negative antigen frequencies are used in the formula below to determine the percent of type-specific units that would be compatible for the patient. Negative antigen frequencies are determined by subtracting the percent antigen frequency from 100%. The negative antigen frequency for K in this case is .91 (1.00 - .09) and the negative antigen frequency for Fya is .34 (1.00 - .66).
% compatible units available = 100 x (Neg frequency #1 x Neg frequency #2...) For this case, the calculation is:
% compatible units available = 100 x (0.91 x 0.34) = 30.94 or 31%
If the antigen frequencies for K = 0.09 and Fya = 0.66, what percent of type-specific units would be compatible for a patient with anti-K and anti-Fya?
- A. 100
- B. 43
- C. 31
- D. 92
- E. 10
Correct Answer: C 🗳️
Question #5
Anything that can cause significant turbidity in a blood sample, such as high leukocyte count or lipemia can potentially interfere with the accuracy of a spectrophotometric hemoglobin assay.
Hematology
Which of the following may interfere with the accurate measurement of hemoglobin:
- A. Leukocytosis and lipemia
- B. Heparin
- C. EDTA
- D. Leukocytosis
Correct Answer: A 🗳️
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