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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is using CRISPR-based technology for rapid and sensitive detection of specific bacterial DNA sequences directly from patient samples. This method relies on the ability of the Cas enzyme to:

A) Specifically bind to and cleave target DNA sequences guided by a short RNA molecule.
B) Amplify target DNA sequences using specific primers and thermal cycling.
C) Detect the presence of specific antibodies produced in response to bacterial infection.
D) Reverse transcribe RNA into complementary DNA.

2. A research laboratory is investigating the mechanism of action of a novel antiviral compound. In vitro studies demonstrate that the compound effectively inhibits the replication of a specific enveloped RNA virus. Further analysis reveals that the compound does not interfere with viral entry, genome replication, or protein synthesis. However, electron microscopy of infected cells treated with the compound shows an accumulation of viral glycoproteins within the endoplasmic reticulum and Golgi apparatus, with a significant reduction in the release of infectious virions. The MOST likely target of this antiviral compound is:

A) Host cell membrane fusion machinery
B) Viral RNA-dependent RNA polymerase
C) Viral glycoprotein trafficking and maturation
D) Viral protease responsible for polyprotein cleavage

3. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.
B) Histoplasma capsulatum; amphotericin B followed by itraconazole.
C) Paragonimus westermani; praziquantel.
D) Blastomyces dermatitidis; itraconazole.

4. A patient returning from a mission trip to Africa presents with fever, headache, and a characteristic relapsing pattern of symptoms. Peripheral blood smear reveals the presence of extracellular flagellated protozoa. The MOST likely diagnosis is African trypanosomiasis caused by:

A) Trypanosoma brucei gambiense or Trypanosoma brucei rhodesiense
B) Leishmania donovani
C) Trypanosoma cruzi
D) Plasmodium vivax

5. A patient develops a severe diarrheal illness after consuming raw oysters. Stool cultures grow a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing green colonies. The MOST likely virulence factor contributing to the watery diarrhea is:

A) Cholera toxin-like enterotoxin
B) Heat-labile toxin (LT)
C) Shiga toxin
D) Cytotoxin-associated gene A (CagA)

Solutions: